Figure 4

The influence of CXCR4 knockdown on Wnt target genes and invasion-related genes. (A) The β-Catenin/Tcf transcription reporter assay. Normalized with control reporter plasmid, the relative luciferase activity was demonstrated. **P < 0.01 compared with NC cells; (B) QT–PCR was applied to examine the change of Wnt downstream target genes expression including β-catenin, c-myc, CD44, Vimentin and Slug by the 2^-∆∆Ct method. shCXCR4 inhibited the expression of these genes, while CXCL12 enhanced their expression. *P < 0.05, **P < 0.01 compared with NC cells; (C) Western blot analysis was used to detect β-catenin, Vimentin and MMP-9 protein expression. β-actin was used as a loading control.