Table 1 KRAS status analyzed in tumor tissue and plasma samples by direct sequencing and PNA-PCR assays
Tumor tissue | Plasma | Total | ||
|---|---|---|---|---|
Assay | KRASstatus | (n = 416) | (n = 242) | (n = 658) |
Wild type | 260 (63) | 201 (83) | 461 (70) | |
Mutated | 156 (38) | 41 (17) | 197* (30) | |
Codon 12 | 118 (28) | 30 (12) | 148 (22) | |
Direct sequencing | 12 D | 50 | 13 | 63 |
12 V | 39 | 10 | 49 | |
Other | 29 | 7 | 36 | |
Codon 13 | 38 (9) | 11 (5) | 49 (8) | |
Wild type | 221 (53) | 166 (69) | 387 (59) | |
Mutated | 195 (47) | 76 (31) | 271* (41) | |
Codon 12 | 133 (32) | 56 (23) | 189 (29) | |
PNA-PCR | 12 D | 57 | 26 | 83 |
12 V | 46 | 17 | 63 | |
Other | 30 | 13 | 43 | |
Codon 13 | 62 (15) | 20 (8) | 82 (12) |