Wnt3a expression was concomitant with EMT immunohistochemical features in human colon cancer tissue samples. E-cadherin expression was higher in Wnt3a negative (−) or weak expression (+) colon cancer tissue sections than in strong-expression (++) samples. E-cadherin was mainly localized in membrane of Wnt3a (−)/(+) samples (red arrows) and in cytoplasm of Wnt3a (++) samples (black arrows). Tumor cells in Wnt3a (−)/(+) section did not express vimentin, whereas several tumor cells in Wnt3a (++) section showed expression of vimentin. In Wnt3a (−)/(+) sections, tumor cells displayed weak and only membrane-localized β-catenin expression, whereas tumor cells in Wnt3a (++) section showed nuclear β-catenin accumulation (black arrows), 400×.