Figure 6

Protein expression of factors related to the p53 signaling pathway in U251 cells treated with HATi II. Western blot analysis of p53 and p21 (A) and factors downstream of the p53 signaling pathway (B, C) in U251 cells treated with HATi II. (D) Western blot analysis confirming siRNA-mediated knockdown of Reprimo. (E) The morphological assay indicated that knockdown of Reprimo markedly reduced cell death in a dose-dependent manner in U251 cells treated with 10 or 20 μmol/l HATi II for 48 h. (F) The hoechst 33342 staining assay indicated that knockdown of Reprimo reduced apoptosis in U259 cells treated with 10 μmol/l HATi II for 48 h compared with control cells; **P < 0.01.