Figure 6

HSPA5 is the direct binding protein to DAL-1 which contributes positively to EMT in lung cancer. A, Co-immunoprecipitation and mass spectrometric assay were performed to show the binding proteins to DAL-1. a, 4.1B; b, E-cadherin; c, HSPA5; d, P4HA1; e, Tubulin beta chain; f, 14-3-3ε. B, Endogenous co-immunoprecipitation analysis conformed that HSPA5 was the direct binding protein to DAL-1. C, The function of three designed HSPA5 RNA interference was determined by western blotting in A549 and NCI-H460 cells. D, The expression of EMT markers E-cadherin, N-cadherin and snail were measured in control and HSPA5 knockdown A549 or NCI-H460 cells by western blotting.