Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi’s sarcoma and non-Kaposi’s cases

Cordiali-Fei, Paola; Trento, Elisabetta; Giovanetti, Marta; Lo Presti, Alessandra; Latini, Alessandra; Giuliani, Massimo; D’Agosto, Giovanna; Bordignon, Valentina; Cella, Eleonora; Farchi, Francesca; Ferraro, Carmela; Lesnoni La Parola, Ilaria; Cota, Carlo; Sperduti, Isabella; Vento, Antonella; Cristaudo, Antonio; Ciccozzi, Massimo; Ensoli, Fabrizio

doi:10.1186/s13046-014-0119-0

Journal of Experimental & Clinical Cancer Research

Table 5 Amino–acid substitutions at the hypervariable codon sites of K1 protein of HHV-8

From: Analysis of the ORFK1 hypervariable regions reveal distinct HHV-8 clustering in Kaposi’s sarcoma and non-Kaposi’s cases

Sequence ID	Genotype	Diagnosis	AA codon 44	AA codon 58	AA codon 60	AA codon 62	AA codon 66	AA codon 67	AA codon 68	AA codon 69	AA codon 70	AA codon 71	AA codon 75	AA codon 101
15 K	A	cKS	Ala	Phe	Pro	Asp	Phe	Pro	Val	Thr	Ile	Ser	Thr	Ala
18 K	C	cKS	Thr	Phe	Leu	Gln	Thr	Val	Val	Asp	Phe	Val	Ser	Val
20 K	C	cKS	Thr	Phe	Leu	Gln	Thr	Val	Ala	Arg	Leu	Val	Ser	Arg
21 K	C	cKS	Thr	Phe	Leu	Gln	Thr	Val	Val	Asn	Leu	Ile	Ser	Gly
26 K	A	cKS	Ala	Leu	Leu	Glu	Ser	Pro	Val	Thr	Ile	Ala	Thr	Ala
27 K	C	cKS	Thr	Phe	Leu	Glu	Thr	Val	Val	Asn	Leu	Ile	Ser	Gly
30 K	A	cKS	His	Trp	Leu	Lys	Asn	Ala	Ser	Thr	Ile	Asn	Leu	Ala
33 K	A	cKS	Ala	Phe	Leu	Glu	Phe	Pro	Leu	Thr	Ile	Ala	Thr	Ala
34 K	C	cKS	Glu	Ser	Leu	Gln	Leu	Val	Gly	Thr	Ile	Ile	Val	Gly
36 K	A	cKS	Ala	Leu	Leu	Lys	Thr	Ala	Val	Thr	Ile	Thr	Thr	Ala
37 K	C	cKS	Thr	Phe	Met	Gln	Thr	Val	Phe	Asn	Leu	Ile	Ser	Gly
40 K	A	cKS	Ala	Val	Leu	Glu	Phe	Pro	Val	Thr	Ile	Ala	Thr	Ala
47 K	A	cKS	Ala	Phe	Ala	Glu	Phe	Pro	Val	Thr	Ile	Ala	Thr	Ala
48 K	A	cKS	Ala	Trp	Leu	Arg	Thr	Val	Ala	Thr	Ile	Thr	Thr	Ala
4 K	A	cKS	Thr	Leu	Leu	Thr	His	Gly	Val	Asn	Leu	Thr	Thr	Ala
14 K	A	eMF	Thr	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	His	Leu	Ala
3 K	A	eMF	Ser	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Thr	Thr	Ala
77 K	A	eMF	Ser	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Thr	Thr	Ala
78 K	A	eMF	Ser	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Thr	Thr	Ala
13 K	C	MF	Ser	Phe	Leu	Arg	Thr	Val	Val	Asn	Ile	Ser	Leu	Gly
17 K	A	MF	Pro	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Asn	Leu	Ala
42 K	C	MF	Thr	Leu	Leu	Lys	Thr	Val	Val	Thr	Ile	Ile	Ser	Ala
5 K	C	MF	Thr	Leu	Leu	Lys	Thr	Val	Val	Thr	Leu	Ile	Ser	Gly
1 K	A	Asympt.	Thr	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Asn	Leu	Ala
2 K	A	Asympt.	Ser	Trp	Leu	Lys	Thr	Ile	Asp	Ile	Ile	Thr	Thr	Ala
43 K	C	Asympt.	Thr	Leu	Leu	Met	Thr	Val	Asp	Thr	Leu	Ile	Ser	Gly
44 K	C	Asympt.	Thr	Leu	Leu	Lys	Thr	Ile	Val	Thr	Leu	Ile	Ser	Gly

Contingency tables and Chi square test suggest an association of amino-acid substitutions with HHV-8 infection and noKS: 4 sequences (boldface text)/12 vs 0/15 KS, p = 0.03 (Fisher).

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ISSN: 1756-9966

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