Figure 4

TFCP2 positively regulates FN1 and negatively regulates TPJ1 in human HCC cells. FN1 and TJP1 protein expression was detected in HepG2 and BEL-7402 cells after TFCP2-knockdown (treated with siTFCP2 for 48 h) and in Hep3B and SK-HEP-1 cells after TFCP2-overexpression (A) by Western blot analysis. Other markers of EMT (E-cadherin, N-cadherin, slug, and snail) were not obvious regulated by TFCP2 (B).