Figure 3
The effects of inflammatory E-cadherin + DCs on Th17 and Treg cells. (A) The polarisation of Th17 cells with the percentage of CD4 + IL-17+ T cells and polarisation of Treg cells with the percentage of CD4 + Foxp3+ T cells. Percentage of Foxp3 + CD4+ cells and IL-17 + CD4+ cells from the E-cadherin + DCs and E-cadherin- DCs groups, according to the gating of CD4 + T cells. (B) Cytokine secretion was measured in the supernatant using the FlowCytomix kit (eBioscience). The supernatant harvested at 72 h and 96 h was used to assess IL-17 secretion, and the supernatant harvested at 72 h and on day 5 was used to assess IL-10 and TGF-β secretion. Each experiment was performed in triplicate. An asterisk indicates a significant difference between the two test groups, as analysed by Student’s t-test (*P < 0.05, ***P < 0.001).