Table 1 Primers used in the construction of the p2KO ablation/insertion vector

left flank

66R L SacI (f)

5′-AATGGATCACATAAAGGAGCTCTTAACG-3′

66R L NotI (r)

5′- CAGAAAACATGCGGCCGCATATAATCT-3′

right flank

66R R EcoRI

5′-GGAGATGAACAAGAAATAGAATTCATAGG-3′

66R R HindIII

5′- CTGTTCTTTATCACAAGCTTCTATCGGGTG-3′

mGM-CSF

hmGMCSF BamHI (f)

5′-TAGGCCTGGGATCCGATCCACCGGTCGCCACCATGTGGCTGCAGA-3′

mGMCSF XmaI (r)

5′-CTCATCAATGTATCTTATCATCCCGGGCTAGCT-3′

mCCL2/MCP-1

mMCP-1 BamHI (f)

5′-TAGGCCTGGGATCCGATCCACCGGTCGCCACCATGCAGGTCCCTG-3′

mMCP-1 XmaI (r)

5′-CGGCGATCCCCGGGAGATACTAGTTCAC-3′

fliC (S. typhimurium)

FliC BamHI (f)

5′-ACCCGGGGATCCTCTAGAAATAATTTTG-3′

FliC XmaI (r)

5′-GGAGCTCGAACCCGGGTCCTTAAC-3′

M13 (f)

5′-TGTAAAACGACGGCCAGT-3′

M13 (r)

5′-CAGGAAACAGCTATGACC-3′

66R ablation verification

66R int (f)

5′- CGGTATCAAATTGCTAGGTATACTTGC-3′

66R int (r)

5′- CTCCAATTCGTTTAGAAAACGATGCG-3′

2L ablation verification

2L int (f)

5′- CCATTGCATCCTTCAGAACAAG-3′

2L int (r)

5′- GCATAACTTTAAAATATAATTATACTGTTACG-3′

PCR template control

136R int (f)

5′- GTATTTATGTACTGTTTCAACTAACAAAAGC-3′

136R int (r)

5′- CCTTTAGGTGTTAGGATATATCAATTATACAG-3′