Figure 2

Methylation analysis of IGFBP-7 promoter and effect of 5′AZA on HNSCC cell lines. (A) Illustration of 5′ region of IGFBP-7. Position of CpG islands and primers for IGFBP7 methylation assay. (B) MSP assay for A253 and its invasive subpopulations. M indicated methylated amplicon and U indicated unmethylated amplicon. (C) BGS of 5′ region in IGFBP-7 for A253 and its invasive subpopulations. Data are shown with an average methylation percentage with or without 5′AZA treatment for A253 cells. (D) Restored IGFBP7 mRNA expression after 5′AZA treatment. IGFBP-7 mRNA expression was detect using Q-PCR after treatment with or without 5′AZA in HNSCC cell lines. *** indicated p < 0.001.