Figure 4
Inactivate of AKT/GSK3β and reduce translocation of β-catenin in IGFBP-7 overexpression A253-5 cells. (A) Western blot analyzed the phosphorylation of AKT (Thr 308) and GSK3β (Ser 9). (B) Western blot using protein from nuclear fraction is analyzed. C: A253-5, Mock: A253-5 transfected with pEGFP-N1, IGFBP7: Transient IGFBP-7 expression A253-5 cells, IGFBP7 + NCsi: Transient IGFBP-7 expression A253-5 cells transfected with negative control siRNA, IGFBP7 + siRNA: Transient IGFBP-7 expression A253-5 cells transfected with siRNA against IGFBP7. *** indicated p < 0.001 compared with A253-5 Mock cells. # indicated p < 0.01 compared with IGFBP7 + NCsi.