Figure 1
From: ARE/SUZ12 dual specifically-regulated adenoviral TK/GCV system for CML blast crisis cells
SUZ12 and Nrf2 status in blast crisis CML cells (A) Structures of Ad luciferase reporter (Ad-AS-luc and Ad-S-luc) and Ad TK vectors (Ad-AS-TK and Ad-S-TK). The ARE construct contains 2 AREs from human NQO1 modifier subunit, and the SUZ12 promoter contains a 2.3 kb upstream fragment. (B) Relative SUZ12 and Nrf2 mRNA of mononuclear cells from five normal bone marrow and six blast phase samples, by RealTime RT-PCR. NBM: normal bone marrow; BP: blast phase. (C) Relative SUZ12 and Nrf2 mRNA in K562, KCL22, K562/G01, and HepG2 cells by RealTime RT-PCR analysis. Data are depicted as relative to actin mRNA, respectively. *, P < 0.05; **, P < 0.01; ***, P < 0.001.