Figure 2

SULF2 enzymatic activity in prostate cancer cells. GAGs labeled with [³⁵S]Na₂SO₄. were purified from the culture medium (MEDIUM), the cancer cells (CELL) extracted with EDTA, and the matrix (MATRIX) produced by cells. The content of GAGs from these compartments was analyzed through agarose gel electrophoresis. The gel was exposed to a radiosensitive screen and quantification was performed by densitometry with Opti-Quanti Software (A). Each sample was digested using a mixture of heparin lyases and analyzed on a PhenoSphere™ 5 μm SAX 80 Å LC Column 150 × 4.6 mm. Δ-disaccharide were eluted with a linear gradient of NaCl 0–1 M over a 30-min period at a flow rate of 1 ml.min^-1. Individual fractions (0.5 ml) were collected and counted using a micro-beta counter (B). The bars indicate the average of three independent experiments. The arrows show the trisulfated disaccharide UA(2S)-GlcNS(6S). *P ≤ 0.05.