Figure 7
Western blot detection of cell cycle-related protein expression. Forty-eight hours after 7901/DDP cells were transfected with miR-223 inhibitor or pcDNA/FBXW7, cells were collected. Western blot detection of cell cycle-related proteins (p14, p16, p21, p27, CDK2, CDK4, CDK6, c-myc, CCND1, CCND2, CCND3, CCNE1 and CCNE2). β-actin protein was used as an internal control. Data are expressed as the mean ± S.D. of three individual experiments.