Figure 1

Evaluation of the functional GJIC between breast cancer-derived T47D cells and AT-MSC based on Calcein AM transfer. Tumor cells were stained with DiI, AT-MSC were stained with Calcein AM. A: T47D cells and AT-MSC were mixed immediately before measurement. B: T47D cells and AT-MSC cocultured for 24 h. R1: DiI-positive T47D cells; R2: DiI-positive T47D cell which received Calcein AM from AT-MSC via gap junctions; R3: Calcein AM-positive AT-MSC;