Suppression of tumor growth following mBAP31 DNA vaccine vaccinations in B16 melanoma-bearing C57BL/6 mice. C57BL/6 mice were transplanted subcutaneously with 5 × 104 B16 cells per mouse at day 0, and were immunized with p-mBAP31, p-LAMP/mBAP31 or PBS on days 3, 10, 17 and 24. Tumor size was serially measured and mice were sacrificed on day 42. Student t test was done based on the data of day 42. *, P < 0.05 for the comparison of the tumor volume between the p-LAMP/mBAP31 vaccinated mice and those vaccinated with p-mBAP31 or PBS. **, P < 0.05 for the comparison between the p-mBAP31 vaccinated mice and those vaccinated with PBS.