Figure 3
Deletion of FoxM1 gene down-regulated the expression of FoxM1 in SW620 cells by shRNA. Western blotting (A-B) and qRT-PCR analyses (C) showed that the level of FoxM1 expression was remarkably decreased in stably FoxM1-depleted SW620 cells at the mRNA and protein levels, respectively. β-actin as a loading control. Each experiment was repeated three times.