Fig. 7

miR-29b directly targets 3’-UTR of the PTEN gene. a, Predicted binding sites in the 3’-UTR of PTEN mRNA and seed sequence of miR-29b by TargetScan. b, Schematic diagrams of miR-29b and PTEN 3’ UTR binding and wild-type and mutated psiCHECK-2-PTEN-3’UTR sequences. c, Relative luciferase activity evaluated by dual luciferase reporter genes following co-transfection with psiCHECK-2-Wt-PTEN-3’UTR or psiCHECK-2-Mut1-3-PTEN-3’UTR and miR-29b mimic, respectively. Results are presented as means ± SEM (*P < 0.05, n = 3). d, Western blots analysis of PTEN expression levels in A549 and H460 cell lines following LV-miR-29b or miR-29b inhibitor infection, respectively. e, Quantitative RT-PCR analysis of PTEN mRNA expression levels in A549 and H460 cell lines following LV-miR-29b or miR-29b inhibitor infection, respectively