Fig. 1
From: Small nucleolar RNA 78 promotes the tumorigenesis in non-small cell lung cancer
SNORD78 is transcribed independently of GAS5. (a) Schematic diagram of SNORD78 and its location in GAS5. GAS5 (top) consists of 12 exons and SNORD78 resides in intron 7–8 of GAS5. SNORD78 is depicted as the gray box. The location of the forward (F) and reverse (R) primers used for RT-PCR are shown. Orientation of arrows indicates the direction of the transcription or amplification reaction. (b) Linear correlation between the expression of the SNORD78 and GAS5 was not observed. ΔCt values were used to measure gene expression, which was normalized by β-actin expression levels. (c) Expression of GAS5 was decreased, and SNORD78 was not significantly changed in A549 and SPC-A1 cells with two different siRNAs against GAS5. Statistical differences were analyzed relative to siRNA control. *, p < 0.05