Fig. 2

Differences in adhesion and gap junction molecule expression of HOSS1 cells in 3D and 2D cultures. a: Immunofluorescence staining of Cx26, Cx43, and Cx45 in 3D (upper) and 2D (lower) cultures of HOSS1 cells. Nuclei were counterstained with DAPI. Bar = 20 μm. Magnification, ×400. b, c: RT-qPCR and western blot analyses revealed up-regulation of the mRNA and protein expression of connexins in 3D cultures, respectively. d, e: mRNA expression of N-cadherin (d) and E-cadherin (e) in HOSS1 cells as determined by RT-qPCR. f: Western blotting showed up-regulation of E-cadherin in a time-dependent manner in 3D cultures, whereas N-cadherin expression reached a peak day 10 and decreased thereafter. Results represent the mean ± SD of three independent experiments with Student’s t-test