Fig. 3

YY1 inhibites miR-9 expression in melanoma cells. (a) A representation of YY1 motifs scattered throughout the human miR-9-1, miR-9-2 and miR-9-3 loci. Fragment 1 and 2 represents the amplified fragments in subsequent ChIP-q-PCR analysis. (b) ChIP-q-PCR analysis of the YY1 hits on three miR-9 loci in WM1791C cells; (c) Q-PCR analysis of YY1 mRNA and miR-9 level in WM1791C cells treated with si-YY1 or si-control for 48 h; (d) Relative miR-9 expression levels in normal skin tissues, benign nevi and melanoma tissues; (e) The Statistical analysis of the association between miR-9 level and metastasis state (primary and metastasis); (f) The Statistical analysis of the association between miR-9 level and tumor stage (I, II, III and IV); (g) The relative level of miR-9 in melanoma cell lines (WM852, WM1791C, WM8, WM209, FO-1, WM983A, WM793 and Daju) relative to 4 normal tissue controls. For the quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *, p < 0.05; **, p < 0.01