Fig. 4

Overepression of miR-9 inhibits melanoma cell proliferation, migration and invasion. (a) miR-9 level were detected in WM1791C and WM209 cells after treatment with miR-9 mimic (25 nM) or mimic control (25 nM) by RT-qPCR; (b) Cell proliferation assay of WM1791C and WM209 cells after treatment with miR-9 mimic or mimic control by using CCK-8; (c) Cell cycle analysis by propidium iodide (PI) staining of WM1791C and WM209 cells after treatment with miR-9 mimic or mimic control; (d) Wound healing assays of WM1791C (the left panel) and WM209 (the right panel) cells after treatment with miR-9 mimic or mimic control, The relative ratio of wound closure per field was shown in the below; (e) Transwell analysis of WM1791C cells after treatment with miR-9 mimic or mimic control, The relative ratio of invasive cells per field was shown below. For the quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *, p < 0.05; **, p < 0.01