Fig. 5

RYBP is repressed by miR-9 in melanoma cells. (a) A computer prediction of the conserved and mutated binding sites within the 3' UTR of human RYBP mRNAs for miR-9; (b) Relative luciferase activity of the indicated RYBP reporter constructs. Error bars represent the standard deviation obtained from three independent experiments; (c) Immunoblot analysis of RYBP in WM1791C and WM209 cells transfected with mimic control or miR-9 mimics; An unrelated protein GAPDH was used as the control. (d) Immunoblot analysis of RYBP in WM1791C and WM209 cells transfected with si-YY1 or si-control; An unrelated protein GAPDH was used as the control. (e) Immunoblot analysis of RYBP in WM1791C transfected with si-YY1, and miR-9 inhibitor or inhibitor control. An unrelated protein GAPDH was used as the control. For the quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *, p < 0.05; **, p < 0.01