Fig. 8

DZNep/Daunoblasatine combination on survival signal transduction. The cells were processed for the determination of the expression (a) and phosphorylation (b) of erk-1 and 2 evaluated after blotting with an anti-MAPK- and an anti-pMAPK-specific Mab, respectively, as described in ‘Materials and methods’. In the same experimental conditions, the activity (c) of Akt was also analysed with a Western blotting and an in-gel kinase assay, respectively, as described in ‘Materials and methods’. (d) Expression of the house-keeping protein beta-tubulin, used as loading control. The experiments were performed at least three different times and the results were always similar. CTR, untreated; Dauno; DZNep; Dauno/DZNep. (e) Scan of the bands associated with Erk 1/2 and Akt activity was performed with a dedicated software and the intensities of the bands were expressed as arbitrary units (%, mean of three different experiments). Bars, s.e.’s