Fig. 2

The functional analysis of MEG3 in GC cells. a YAP1 level were detected in HGC-27 and MGC-803 cells after treatment with pCMV-MEG3 or pCMV6 empty vector by RT-qPCR; b Cell proliferation assay of HGC-27 and MGC-803 cells after treatment with si-YAP1 or control by using CCK-8; c Flow cytometry was applied to examine the apoptosis of HGC-27 and MGC-803 cells after treatment with pCMV-MEG3 or pCMV6 and stained with apoptosis markers (FITC-Annexin V and PI). In the apoptosis map, FITC-Annexin V⁺/PI⁺ indicates late apoptosis, FITC-Annexin V⁺/PI⁻ indicates early apoptosis, and FITC-Annexin V⁻/PI⁻ indicates normal live cells. d Wound healing assays of HGC-27 and MGC-803 cells after treatment with pCMV-MEG3 or pCMV6, The relative ratio of wound closure per field was shown in the right; e Transwell analysis of HGC-27 and MGC-803 cells after treatment with pCMV-MEG3 or pCMV6, The relative ratio of invasive cells per field was shown right. For all quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *p < 0.05; **p < 0.01