Fig. 3

The interaction of MEG3 with miR-181. a The upper panel: schematic outlining the predicted binding sites of miR-181 s on MEG3. The lower panel: the prediction for miR-181 s binding sites on MEG3 transcript. The red nucleotides are the complementary sequences to miR-181 seed sequences. b Luciferase activity in HGC-27 cells cotransfected with miR-181a mimic (25 nM) mimic control (25 nM) and luciferase reporters containing nothing, wild type MEG3 or diverse mutant MEG3 as indicated. Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. c MS2-RIP followed by miRNA RT-qPCR to detect miR-181a endogenously associated with MEG3. For all quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *p < 0.05; **p < 0.01