Fig. 4

The functional analysis of miR-181a in GC cells. a Relative miR-181a expression level in GC tissues and adjacent normal regions; The expression of level of miR-181a was detected in 50 GC patients by RT-qPCR. b miR-181a level were detected in HGC-27 and MGC-803 cells after treatment with miR-181a inhibitor (25 nM) or inhibitor control (25 nM) by RT-qPCR; c Cell proliferation assay of HGC-27 and MGC-803 cells after treatment with miR-181a inhibitor or inhibitor control by using CCK-8; d Flow cytometry was applied to examine the apoptosis of HGC-27 and MGC-803 cells after treatment with miR-181a inhibitor or inhibitor control and stianed with apoptosis markers (FITC-Annexin V and PI). In the apoptosis map, FITC-Annexin V⁺/PI⁺ indicates late apoptosis, FITC-Annexin V⁺/PI⁻ indicates early apoptosis, and FITC-Annexin V⁻/PI⁻ indicates normal live cells. e Wound healing assays of HGC-27 and MGC-803 cells after treatment with miR-181a inhibitor or inhibitor control, The relative ratio of wound closure per field was shown in the right; f Transwell analysis of HGC-27 and MGC-803 cells after treatment with miR-181a inhibitor or inhibitor control, The relative ratio of invasive cells per field was shown right. For all quantitative results, the data are presented as the mean ± SEM, and the error bars represent the standard deviation obtained from three independent experiments. *p < 0.05; **p < 0.01