Fig. 5

Isobologram showing the in vitro interaction between amantadine and doxorubicin, cisplatin or 5FU in HCT8^WT and HCT8^RETO cells. Dose-response curves are based on the respective IC₅₀ values calculated after 24 hours of incubation. Amantadine-doxorubicin are synergistic in HCT8 ^WT/RETO (a & b). Amantadine-cisplatin are additive in HCT8 wildtype (c) and synergistic in HCT8^RETO (d). Amantadine-5FU are synergistic in RETO (f) but antagonistic in HCT8^WT (e). n = 3 independent experiments