Fig. 3
From: Drug-releasing mesenchymal cells strongly suppress B16 lung metastasis in a syngeneic murine model
Chemotaxis of SR4987 and SR4987PTX induced by mL-StCs-CM upon priming with B16-CM or B16-CM + TNFα mediated by SDF-1/CXCR4/CXCR7 axis. In (a) SR4987PTX and (b) SR4987 cells migration. Bars are the mean ± SD of three independent experiments. Note the chemotactic activity of mL-StCs-B16-CM and mL-StCs-TNFα-B16-CM on both SR4987PTX and SR4987 (*P < 0.05;**P < 0.01 versus CTRL). B16-CM and mL-StCs-CM are not effective while SDF-1 induces SR4987PTX and SR4987 chemotaxis. In (c) SDF-1 secreted by the B16 melanoma cells and by mL-StCs under different stimuli. Bars are the mean value ± SD of SDF-1 detected in the CMs normalized for the same number of cells (106). In (d) the analysis of SDF-1 at mRNA level in B16 and mLStCs under different culture conditions. Bars ± SD are the relative gene expression calculated by a comparative method (2-ΔΔCt) using GAPDH as an housekeeping gene. In (e) SR4987PTX and (f) SR4987 cells migration in the presence of anti-SDF-1 (1 μg/ml) or after cells priming with AMD3100 (1 μM). Note that both anti-SDF-1 and AMD3100 significantly reduce chemiotaxis (° < 0.05 versus no anti-SDF-1 addition; **p < 0.01 versus no AMD3100 treatment)