Fig. 1

ABC294640 induces growth inhibition and apoptosis in CRC cell lines. The relative growth (vs. Control group) of tested CRC cells with indicated ABC294640 treatment was tested by MTT assay (a and b). HT-29 cells, treated with or without applied ABC294640 for indicated time, were subjected to clonogenicity assay (c), Annexin V FACS assay (d), histone-DNA ELISA assay (e) or LDH release assay (f). Mean values ± SD of three independent experiments were reported. Statistical analysis was performed comparing treatment groups with vehicle control group (“C”). *P < 0.05