Fig. 3
From: Resistance to multikinase inhibitor actions mediated by insulin like growth factor-1
Antagonism by IGF1 of regorafenib-mediated inhibition of migration and invasion. PLC/PRF/5 cell line cultured in 1 % FBS medium was treated with IGF1 40 ng/ml alone or in combination with Regorafenib 5 μM and GSK 1 μM. a. Migration assay was performed as described and the microscopic analysis was assessed at the time of the scratch (T0) and after 48 h (T2). The values were expressed as percentage of migration, where 100 % represents the scratch completely closed. b. The percentage of invasion was calculated comparing the invading drug-treated cells to drug-untreated control cells (100 %). The results of three independent experiments are expressed as means ± SD. ***p < 0.0001