Fig. 8

Drug combination studies of cobimetinib with cis-RA in NB cell lines: NB cells were incubated with 1 μM of cis-RA acid plus IC₂₅ concentration of cobimetinib for each cell line as obtained from Fig. 1. The IC₂₅ values used were 2 μM, 0.07 μM and 0.04 μM for IMR-5, SHEP and IMR-32 respectively. Data shown indicate percent survival with each drug or in combination compared to corresponding DMSO treatment. Cultures were set up in triplicate and the data presented are representative of two separate experiments. P values indicate statistical significance. Figures a, b, and c show findings from the cell lines IMR-32, IMR-5 and SHEP respectively