Fig. 3

CD98 directly interacts with basigin in HCC cells. a Co-localization of basigin and CD98 in SMMC7721, HepG2 and Huh7 HCC cell lines. The cells were grown on coverslips for 24 h, fixed and stained with Dylight488-conjugated goat-anti-rabbit antibodies (basigin, Green) and Dylight594-conjugated goat-anti-mouse antibodies (CD98, Red). Huh7 cells were treated with 0.2 % Triton X-100 after fixation to visualize the intracellular distribution of basigin and CD98. Bar, 10 μm. b CD147-ED pulls down CD98-ED in vitro. HAb18 (antibody against CD147-ED; 10 μg) was used as a coupling antibody by immobilizing it onto the coupling resin. Then, the indicated amount of the antigen was added and eluted. The collected samples were analyzed by western blotting. c Biophysical analysis of the interaction of CD98-ED with CD147-ED using SPR. The indicated concentrations of purified CD98-ED were injected over immobilized CD147-ED, and the biophysical parameters were derived from a 1:1 binding model (red line). RU, response units