Fig. 4

The changes of OS cell migration and invasion abilities after treatment with oleandrin. a/b The migration of U2OS and SaOS-2 cells before and after treatment of U2OS cells (a, for 6, 12 and 24 h) and SaOS-2 cells (b, for 24, 48 and 72 h) with 25 nM oleandrin, was determined by a wound healing assay. c/d A graphical representation of the average distance moved by U2OS (c) and SaOS-2 (d) cells in the oleandrin-treated and control groups. e The invasiveness of U2OS and SaOS-2 cells was observed by a transwell invasion assay after treatment with 25 nM oleandrin for 24 h and was compared to the control group. f The number of cells that invaded the substratum of the membrane per view under a 200× magnification. n = 3. Mean ± SD. ^* P < 0.05, ^** P < 0.01, vs. control group (CTL)