Fig. 6

Western blotting and gelatin zymography. a The protein expression levels of the relevant downstream molecules in the Wnt/β-catenin pathway after the treatment of U2OS cells with 50 nM oleandrin for 0, 24 and 48 h as determined by western blotting. b The semi-quantitative results of the relevant downstream molecules in the Wnt/β-catenin pathway relative to the GAPDH protein. ^* P < 0.05, ^** P < 0.01, compared to the 0 h group. ^# P < 0.05, ^## P < 0.01, compared to the 24 h group. c Representative electrophoretograms of the total, nuclear and cytoplasmic β-catenin levels after treatment with 50 nM of oleandrin for 0, 24 and 48 h using western blotting. d The semi-quantitative results of the total, nuclear and cytoplasmic β-catenin levels based on electrophoretograms from the western blot analysis. ^* P < 0.05, ^** P < 0.01, compared to the 0 h group. ^# P < 0.05, ^## P < 0.01, compared to the 24 h group. (e) The MMP-2 and MMP-9 activities after treatment with 25 and 50 nM of oleandrin as detected by a gelatin zymography assay. CP: cytoplasmic protein; NP: nuclear protein; TP: total protein