Fig. 2

Exposure of cells to D11 leads to induction of apoptotic cell death. a Cells were treated with DMSO and increasing concentrations of D11 for 24 h (M059K) and 48 h (MIA PaCa-2), respectively. Whole cell lysate was subjected to Western blot analysis for apoptosis-associated proteins. b Whole cell lysate from cells treated for 24 h with the indicated concentrations were subjected to ROCK kinase assay in the presence of myosin phosphatase target subunit 1 (MYPT1). Data from one representative experiment (mean +/− standard deviation, N = 6, *, P <0.05; **, P <0.001) are shown. c Immunofluorescence analysis of control cells (0.1 % DMSO) or cells treated with the indicated concentrations of D11 for 24 h. Actin filaments were visualized by staining cells with phalloidin-Alexa 488. Representative images were acquired using fluorescence microscopy at 400x magnification