Fig. 5

D11-mediated effects on the transcriptional activity of NF-κB. a Cells were treated as described in Fig. 4 and Additional file 4: Figure S4. Subcellular localization of NF-κB was detected by immunofluorescence staining of cells with rabbit monoclonal anti-NF-κB antibody (green fluorescence, Alexa Fluor 488). Nuclei were visualized by DAPI staining. NC, negative control refers to cells stained with biotinylated secondary antibody and Alexa Fluor 488 streptavidin, only. Original magnification: 400x. b NF-κB transcriptional activity was determined as described in Materials and methods. The indicated cell lines were treated as described in Fig. 4. Asterisks denote statistical significant differences in TNFα-induced NF-κB activity in the absence or presence of D11 (N = 6, *, P <0.05). Experiments were repeated at least three times obtaining similar results