Fig. 3

CMTM4 inhibits 786-O cell growth. a Overexpression of CMTM4 by infection with a CMTM4-expressing adenovirus (Ad-CMTM4) was confirmed by western blotting. The empty adenovirus (Ad-null) was used as a control. The Cell Counting Kit-8 (b) and viable cell counting (c) assays showed that cell growth was inhibited by overexpression of CMTM4 in 786-O cells. The results are expressed as the means ± SEM of three independent experiments in triplicate. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 compared to the controls at each time point. d Knockdown of CMTM4 with two siRNAs (si-CMTM4-3 and 6) in 786-O cells was verified by quantitative RT-PCR (upper panel) and western blotting (lower panel). si-NC, negative control siRNA. The CMTM4 expression levels were measured relative to the level in the si-NC transfected cells in quantitative RT-PCR. The Cell Counting Kit-8 (e) and viable cell counting (f) assays showed that cell growth was promoted by knockdown of CMTM4 in 786-O cells. The results are expressed as the means ± SEM of three independent experiments in triplicate