Fig. 4

Loss of NAV2 expression decreased the phosphorylation of SSH1L but increased the phosphorylation of cofilin-1. a The expressions of p-SSH1L, SSH1L, p-cofilin-1 and cofilin-1 were detected by Western blotting analysis. Level of p-SSH1L and p-cofilin-1 protein in shRNA and empty vector HCT-116 (Student’s t-test, *P < 0.0001, ***P = 0.0006); Level of p-SSH1L and p-cofilin-1 protein in shRNA and empty vector LoVo cells (Student’s t-test, **P < 0.0001 ****P < 0.0001). Experiments have been repeated three times. b F-actin polymerization and stress fiber disassembly decreased in NAV2-depleted HCT-116/LoVo cells. Experiments have been repeated three times. c The expressions level of p-SSH1L, SSH1L, p-cofilin-1 and cofilin-1 in CRC tissues with different NAV2 expression level were detected by Western blotting analysis