Fig. 2

ERα decreased MTA1 expression in HCC cells. a Hep3B cells were maintained in phenol red-free DMEM with 10 % dextran-coated charcoal-treated FBS for 48 h and cells treated with either ethanol vehicle or E2 (10 or 100 nM) for 72 h. Cells were harvested and analyzed for MTA1 mRNA (right) and protein. Results were from three independent experiments and presented as mean ± SEM. b Western blot and real-time PCR analysis (right) for MTA1 protein and mRNA in Hep3B cells with MOI 20 or 60 ERα lentivirus infection. Results were from three independent experiments and presented as mean ± SEM. c Western blot and real-time PCR analysis (right) for MTA1 protein and mRNA in HepG2 cells with MOI 20 or 60 for shRNA-ERα lentivirus infection (n = 3, mean ± SEM) *P < 0.05, **P < 0.01 by t-test