Fig. 4

Restoration of elevated MTA1 by ectopic expression abrogated ERα-mediated suppression of proliferation and invasion. a Western blots showed that Hep3B-ERα/MTA1 cells exhibited ERα and MTA1 ectopic expression after ERα or MTA1 lentivirus infection. b MTA1 overexpression increased growth of Hep3B-ERα/MTA1 compared to Hep3B-ERα cells. Absorbance at 450 nm was measured. Results were from three independent experiments and presented as mean ± SEM. c Bright cyan, EdU-positive. EdU assays showed that restoration of MTA1 increased the percent of EdU-positive cells compared to Hep3B-ERα cells (n = 3, mean ± SEM). d Representative images of transwell invasion assays. MTA1 overexpression increased invasion by Hep3B-ERα/MTA1 cells compared to Hep3B-ERα cells (n = 3, mean ± SEM). e Macroscopic images (left) and weights of subcutaneous tumors (right) on day 28 after s.c.-administered Hep3B-ERα/MTA1, Hep3B-ERα and control cells. Data are mean ± SEM of tumor weights (n = 6 each). *P < 0.05 by t-test