Fig. 4

EGFR was identified as a binding target of Linc00152. a RNA pull-down experiment with MGC803 extract in different groups. The left two bands indicated Linc00152 wide type while the right two bands presented the antisense of Linc00152. b Mass spectrometry identification of target band of EGFR. The secondary ion mass spectrometry with the peptide sequence labeled was presented. c, d RIP assay was performed using EGFR antibody and was validated by agarose electrophoresis by using different primer. The band from the top to bottom presented DNA marker, RNA extracted with EGFR antibody treating, input (regard as a positive control) and IgG (negative control), respectively. Fold change enrichment was calculated comparing with the input in panel d. Data was presented with mean ± SEM