Fig. 1

Effect of Vernodalin on modulation of FOXO3a and Bim. Expression of FOXO3a/p-FOXO3a (Ser253) and Bim in breast cancer cells of MCF-7 (a) and MDA-MB231 (b). Whole cell lysates were prepared from the breast cancer cell lines after the vernodalin treatment for 24 h. The expression of FOXO3a, phosphorylated FOXO3a, and Bim were analysed by Western blots as described in “Materials and Methods”. β-actin was used as loading control. The data are representative of three experiments. Respective blots were quantified using ImageJ software. Data’s are expressed as mean ± S.D. All the treatment groups were compared with control. “*” denotes statistically significant at P < 0.05