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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Forkhead Box Transcription Factor (FOXO3a) mediates the cytotoxic effect of vernodalin in vitro and inhibits the breast tumor growth in vivo

Fig. 4

Effect of FOXO3a silencing during vernodalin treatment in MCF-7 and MDA-MB231 cells. a Cells were pre-treated with scrambled siRNA or siFOXO3a for 48 h, followed by vernodalin (9.5 μg/ml) treatment for 24 h in MCF-7 and MDA-MB231 cells. All groups of cells were analysed by using Western blots. b & c Cells were fixed in ethanol and stained with propidium iodide, and then the percentage of apoptotic cells was determined by flow cytometry. Data are presented as means ± SD of three independent experiments (*P < 0.05). d & e Cells were pre-treated with scrambled siRNA or siFOXO3a for 48 h, followed by vernodalin (9.5 μg/ml) treatment for 24 h in MCF-7 and MDA-MB231 cells. Protein lysates were prepared at the times indicated and the levels of p27kip1, p21cip1/waf1 and cyclin D1 were analysed by using Western blots. Data are presented as means ± SD of three independent experiments

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