Fig. 6

Effect of vernodalin treatment on proliferation markers (Ki-67 and PCNA) in breast tumor tissue. Sprague dawley rats were implanted with LA7 cells. Rats were treated with low and high dose of vernodalin (1 or 10 mg/kg) and the standard drug Tamoxifen (1 mg/kg). Tumor tissues were collected in 10 % formalin and blocks were prepared in paraffin and immunohistochemistry of PCNA and Ki67 were performed as described in “Materials and Methods”. Photomicrographs show representative pictures from five independent tumor samples. Bar =10 μm. Quantification of Ki-67 and PCNA positive cells in tumor tissues was shown (*P < 0.05)