Fig. 2

Effect of DHA + VitE, DHA and DHA + H2O2 on the growth of breast carcinoma cell line MDA-MB-231 (a) and normal immortalized breast epithelial cells MCF-10A (b). The cells were exposed to 0.3, 1, 3, 10, 30, 50, 100 μM and after 4 days, cell proliferation was measured by MTT 0.5 mg/mL method (see Materials and methods). The relative cell proliferation (absorbance of test wells) was expressed as a percentage of the control (100 %) that was not treated with DHA (absorbance of control wells without DHA or with NC alone). Points are expressed as the mean values relative to control for three separate experiments, each set up in triplicate wells