| | | |
Before fractionation
| | |
After fractionation
| |
|---|
|
Formulations
|
AFM
|
DLS
|
Mean
|
Mean ζ Zeta
|
AsF4-DLS
|
AsF4-MALLS
|
Rg/Rh
|
| |
mean size
|
Z-average size
|
PdI
|
Potential ± SD (mV)
|
Z-average
|
Dg (nm)
|
(shape factor)
|
| |
± SD (nm)a
|
Dh ± SD (nm)
| | |
Dh (nm)
| | |
|
NC PCL
|
193 ± 28
|
157 ± 3
|
0.122
|
−28.9 ± 1.4
|
124 ± 9
|
111 ± 1
|
1.152
|
|
NC PCL-DHA
|
183 ± 26
|
161 ± 1
|
0.113
|
−29.7 ± 0.7
|
145 ± 31
|
116 ± 7
|
1.032
|
|
NC PCL-DHA + Vit E
|
203 ± 42
|
179 ± 3
|
0.089
|
−20.6 ± 0.7b
|
170 ± 25
|
128 ± 15
|
0.976
|
|
NC PCL-DHA + H2O2
|
236 ± 59
|
207 ± 2b, c
|
0.160
|
−32.4 ± 1.6c
|
144 ± 31
|
205 ± 13c
|
1.838c
|
|
NC PCL-4F4t
|
194 ± 52
|
161 ± 3
|
0.111
|
−29.5 ± 1.6
|
120 ± 18
|
112 ± 9
|
1.218
|
-
ζ Zeta Potential, AFM atomic force microscopy, AsF4 asymmetrical flow-field-flow- fractionation, DLS dynamic light scattering, PdI polydispersity index, Dh z- verage hydrodynamic diameter, Dg mean geometric diameter (Dg = 2*Rg*0.775), LDA Laser Doppler Anemometry, MALLS Multi Angle Laser Light Scattering Detector, PεCL poly-ε-caprolactone, Rg radius of gyration (root mean square radius), Rh hydrodynamic radius, EE encapsulation efficiency, PdI polydispersity index, Dh z-average hydrodynamic diameter, Dg mean geometric diameter (Dg = Rg*2*0.775), Rg radius of gyration (root mean square radius), Rh hydrodynamic radius. adetermined by the measurements of at least 40 particles in the height images; mean values taken from the selection of the most intense peak in the fractogram. Shape factor calculation (Mathaes et al., 2013). bSignificant difference (p <0.05) from control formulation; c Significant difference (p <0.05) from non-oxidized
