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Table 1 Physicochemical characterization of the PUFA nanocapsules using different methods before and after sample fractionation

From: Polymeric nanocapsules prevent oxidation of core-loaded molecules: evidence based on the effects of docosahexaenoic acid and neuroprostane on breast cancer cells proliferation

    Before fractionation    After fractionation  
Formulations AFM DLS Mean Mean ζ Zeta AsF4-DLS AsF4-MALLS Rg/Rh
  mean size Z-average size PdI Potential ± SD (mV) Z-average Dg (nm) (shape factor)
  ± SD (nm)a Dh ± SD (nm)    Dh (nm)   
NC PCL 193 ± 28 157 ± 3 0.122 −28.9 ± 1.4 124 ± 9 111 ± 1 1.152
NC PCL-DHA 183 ± 26 161 ± 1 0.113 −29.7 ± 0.7 145 ± 31 116 ± 7 1.032
NC PCL-DHA + Vit E 203 ± 42 179 ± 3 0.089 −20.6 ± 0.7b 170 ± 25 128 ± 15 0.976
NC PCL-DHA + H2O2 236 ± 59 207 ± 2b, c 0.160 −32.4 ± 1.6c 144 ± 31 205 ± 13c 1.838c
NC PCL-4F4t 194 ± 52 161 ± 3 0.111 −29.5 ± 1.6 120 ± 18 112 ± 9 1.218
  1. ζ Zeta Potential, AFM atomic force microscopy, AsF4 asymmetrical flow-field-flow- fractionation, DLS dynamic light scattering, PdI polydispersity index, Dh z- verage hydrodynamic diameter, Dg mean geometric diameter (Dg = 2*Rg*0.775), LDA Laser Doppler Anemometry, MALLS Multi Angle Laser Light Scattering Detector, PεCL poly-ε-caprolactone, Rg radius of gyration (root mean square radius), Rh hydrodynamic radius, EE encapsulation efficiency, PdI polydispersity index, Dh z-average hydrodynamic diameter, Dg mean geometric diameter (Dg = Rg*2*0.775), Rg radius of gyration (root mean square radius), Rh hydrodynamic radius. adetermined by the measurements of at least 40 particles in the height images; mean values taken from the selection of the most intense peak in the fractogram. Shape factor calculation (Mathaes et al., 2013). bSignificant difference (p <0.05) from control formulation; c Significant difference (p <0.05) from non-oxidized