| | |
Before fractionation
| | |
After fractionation
| |
---|
Formulations | AFM | DLS | Mean | Mean ζ Zeta | AsF4-DLS | AsF4-MALLS | Rg/Rh
|
| mean size | Z-average size | PdI | Potential ± SD (mV) | Z-average | Dg (nm) |
(shape factor)
|
| ± SD (nm)a
| Dh ± SD (nm) | | | Dh (nm) | | |
NC PCL | 193 ± 28 | 157 ± 3 | 0.122 | −28.9 ± 1.4 | 124 ± 9 | 111 ± 1 | 1.152 |
NC PCL-DHA | 183 ± 26 | 161 ± 1 | 0.113 | −29.7 ± 0.7 | 145 ± 31 | 116 ± 7 | 1.032 |
NC PCL-DHA + Vit E | 203 ± 42 | 179 ± 3 | 0.089 | −20.6 ± 0.7b
| 170 ± 25 | 128 ± 15 | 0.976 |
NC PCL-DHA + H2O2 | 236 ± 59 | 207 ± 2b, c
| 0.160 | −32.4 ± 1.6c
| 144 ± 31 | 205 ± 13c
| 1.838c
|
NC PCL-4F4t | 194 ± 52 | 161 ± 3 | 0.111 | −29.5 ± 1.6 | 120 ± 18 | 112 ± 9 | 1.218 |
-
ζ Zeta Potential, AFM atomic force microscopy, AsF4 asymmetrical flow-field-flow- fractionation, DLS dynamic light scattering, PdI polydispersity index, Dh z- verage hydrodynamic diameter, Dg mean geometric diameter (Dg = 2*Rg*0.775), LDA Laser Doppler Anemometry, MALLS Multi Angle Laser Light Scattering Detector, PεCL poly-ε-caprolactone, Rg radius of gyration (root mean square radius), Rh hydrodynamic radius, EE encapsulation efficiency, PdI polydispersity index, Dh z-average hydrodynamic diameter, Dg mean geometric diameter (Dg = Rg*2*0.775), Rg radius of gyration (root mean square radius), Rh hydrodynamic radius. adetermined by the measurements of at least 40 particles in the height images; mean values taken from the selection of the most intense peak in the fractogram. Shape factor calculation (Mathaes et al., 2013). bSignificant difference (p <0.05) from control formulation; c Significant difference (p <0.05) from non-oxidized