Fig. 5
Artificial hTERT promoter-Bax-Anti Bcl2 combination module selectively induced cell apoptosis in bladder cancer cells. The cell apoptosis changes were determined by caspase-3 ELISA assay and flow cytometry analysis. The relative activity of caspase-3 was increased in bladder cancer 5637 (a) cells and T24 (b) cells treated with artificial hTERT promoter-Bax-Anti Bcl2 combination module. Cell apoptosis induction were also observed in bladder cancer 5637 (d) cells and T24 (e) cells treated with artificial hTERT promoter-Bax-Anti Bcl2 combination module using flow cytometry analysis. Representative images of flow cytometry analysis in bladder cancer 5637 cells (g) and T24 cells (h) were also shown. Nevertheless, there was no obvious difference with the relative activity of caspase-3 (c) and cell apoptosis induction (f, i) between artificial hTERT promoter-Bax-Anti Bcl2 combination module and negative control in NHF. D1, cell debris. D2, non-viable apoptotic cell. D3, normal cells. D4, viable apoptotic cell. All data are presented as mean ± SD