Fig. 4

Lung metastasis was reduced in JUNB-knockout KCC-T871 cells in the experimental lung metastatic mouse model. a Survival time of the control group with KCC-T871/crControl (N = 14) and JunB knockout (KO) group with KCC-T871/JUNB/KO#1 (N = 16). The survival rate for the control group was significantly lower than that for the JunB KO group. ***P < 0.001. b Median lung weight of the control and JunB KO groups. Median lung weight of mice in the control group was significantly heavier than for that for the JunB KO group. P = 0.001, Boxes, 25th, 50th and 75th percentiles; bars, 10th and 90th percentiles. c Hematoxylin and eosin (H&E) slides of the lung from a mouse injected with KCC-T871/crControl cells in the locations marked with asterisks (*). d H&E slides of the lung in a mouse injected with KCC-T871/JUNB/KO#1 cells in the locations marked with asterisks (*). e Median lung weight of the control group (N = 6) and JunB KO group (N = 8) in the repeated animal study. JunB knockout reduced lung weight in the animal model; however, the difference was not significant. P = 0.0727, Boxes, 25th, 50th and 75th percentiles; bars, 10th and 90th percentiles. f The area occupied by metastatic HNSCC cells in the lung in an experimental lung metastatic mouse model. The mean percentage of the lung area displaying metastatic HNSCC cells in the JunB KO group (N = 24) was significantly reduced compared to that in the control group (N = 18). **P < 0.01, Boxes, 25th, 50th and 75th percentiles; bars, 10th and 90th percentiles