Table 1 Sequences of CRISPR sgRNA and confirming primers used in this study
Name | sgRNA sequence (5′–3′) |
Control (forward) | CATTTCTCAGTGCTATAGAGTTTT |
Control (reverse) | TCTATAGCACTGAGAAATGCGGTG |
JUNB#1 (forward) | GTCTCTCAAGCTCGCCTCTTGTTTT |
JUNB#1 (reverse) | AAGAGGCGAGCTTGAGAGACCGGTG |
JUNB#2 (forward) | GCATCAAAGTGGAGCGCAAGGTTTT |
JUNB#2 (reverse) | CTTGCGCTCCACTTTGATGCCGGTG |
| Â | confirming primers |
JUNB#1 (forward) | TGGAACAGCCCTTCTACCAC |
JUNB#1 (reverse) | TGCTGAGGTTGGGTGTAAACG |
JUNB#2 (forward) | CGACCACCATCAGCTACCTC |
JUNB#2 (reverse) | AGAAGGCGTGTCCCTTGAC |